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The biological test methods and guidance documents below have been prepared by the Method Development and Application Section (MDAS)of the Biological Methods Division. Section staff are responsible for developing, validating and standardizing toxicology test methods required for Environment Canada programs. Since 1990, 18 standardized toxicity test methods and 5 supporting guidance documents have been published and distributed to a national and international audience. Most of the published methods deal with the testing of industrial effluents, surface waters and freshwater or marine sediments and have been developed with the full cooperation of staff from EC regional, provincial, and private-sector laboratories. Currently, MDAS’s focus is on the standardization of toxicology test methods for the assessment of contaminants and substances in soils as well as provision of further guidance on the use of statistics in the determination of toxicity test endpoints.
The Section provides guidance on toxicological data interpretation and program application of this type of data in Fisheries Act and the Canadian Environmental Protection Act (CEPA) regulations and guidelines. The Section also provides technical support in the area of environmental toxicology to the national laboratory accreditation program managed by the Canadian Association of Environmental Analytical Laboratories (CAEAL).
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Biological Test Method:
Acute Lethality Test Using Rainbow Trout |
EPS1/RM/ 9 |
July 1990 (with May 1996 amendments) |
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Biological Test Method:
Acute Lethality Test Using Threespine Stickleback (Gasterosteus aculeatus) |
EPS1/RM/ 10 |
July 1990
Including March 2000 Amendments |
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Biological Test Method:
Acute Lethality Test Using Daphnia spp. |
EPS1/RM/ 11 |
July 1990 (with May 1996 amendments) |
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Guidance Document on Control of Toxicity Test Precision Using Reference Toxicants |
EPS1/RM/ 12 |
August 1990 |
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Biological Test Method:
Reference Method for Determining Acute Lethality of Effluents to Rainbow Trout |
EPS1/RM/13 |
SECOND EDITION
December 2000 |
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Biological Test Method:
Reference Method for Determining Acute Lethality of Effluents to Daphnia magna |
EPS1/RM/14 |
SECOND EDITION
December 2000 |
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Biological Test Method:
Test of Reproduction and Survival Using the
Cladoceran Ceriodaphnia dubia |
EPS1/RM/21 |
February 1992
(Including November 1997 Amendments) |
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Biological Test Method:
Test of Larval Growth and Survival Using Fathead Minnows |
EPS1/RM/22 |
February 1992
(Including November 1997 Amendments) |
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Biological Test Method:
Toxicity Test Using Luminescent Bacteria |
EPS1/RM/24 |
November 1992 |
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Biological Test Method:
Growth Inhibition Test Using the Freshwater Alga Selenastrum capricornutum |
EPS1/RM/25 |
November 1992
(Including November 1997 Amendments) |
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Biological Test Method:
Acute Test for Sediment Toxicity Using Marine or Estuarine Amphipods |
EPS1/RM/26 |
December 1992
(Including October 1998 Amendments) |
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Biological Test Method:
Fertilization Assay Using Echinoids
(Sea Urchins and Sand Dollars) |
EPS1/RM/27 |
December 1992
(Including November 1997 Amendments) |
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Biological Test Method:
Toxicity Tests Using Early Life Stages of Salmonid Fish (Rainbow Trout) |
EPS1/RM/28 Second Edition |
July 1998 |
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Guidance Document on Collection and Preparation of Sediments for Physicochemical Characterization and Biological Testing |
EPS1/RM/29 |
December 1994 |
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Guidance Document on Measurement of Toxicity Test Precision Using Control Sediments Spiked with a Reference Toxicant |
EPS1/RM/ 30 |
September 1995 |
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Biological Test Method:
Test for Survival and Growth in Sediment Using Larvae of Freshwater Midges (Chironomus tentans or
Chironomus riparius ) |
EPS1/RM/32 |
December 1997 |
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Biological Test Method:
Test for Survival and Growth in Sediment Using the Freshwater Amphipod
Hyalella azteca |
EPS1/RM/33 |
December 1997 |
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Guidance Document on Application and
Interpretation of Single-species Tests in Environmental Toxicology |
EPS1/RM/34 |
December 1999 |
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Biological Test Method: Reference Method for Determining Acute Lethality of Sediment to Marine or Estuarine Amphipods |
EPS1/RM/35 |
December 1998 |
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Biological Test Method:
Test for Measuring the Inhibition of Growth Using the Freshwater Macrophyte,
Lemna minor |
EPS1/RM/37 |
March, 1999 |
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Biological Test Method: Test for Survival and Growth in Sediment Using Spionid Polychaete Worms (Polydora cornuta) |
EPS1/RM/41 |
December 2001 |
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Biological Test Method: Reference Method for Determining the Toxicity of Sediment Using Luminescent Bacteria in a Solid-Phase Test |
EPS1/RM/42 |
April 2002 |
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Guidance document for testing the pathogenicity and toxicity of new microbial substances to aquatic and terrestrial organisms |
EPS1/RM/44 |
March 2004 |
